Study: Maintaining the enzyme ACE2 in the gut prevents diabetic blindness

Maria Grant, MD, leader of the research team and a professor in the University of Alabama at Birmingham Department of Ophthalmology and Visual Sciences.

A leading cause of blindness in American adults is diabetic retinopathy, and investigators believe the source of this damage may lie in the belly — mainly a leaky small intestine that weakens the barrier between gut bacteria and the blood system, according to a study published in the journal Circulation Research.1

According to a University of Alabama at Birmingham (UAB) news release, the research blood from human subjects with Type 1 diabetes and a mouse model of Type 1 diabetes were used to explore mechanisms underlying diabetic retinopathy. The results show a way to possibly prevent, or even reverse, the eye damage.

“To our knowledge, this study represents the first time that gut barrier disruption has been implicated in the pathogenesis of diabetic retinopathy and also directly links gut leakage with retinopathy severity in human subjects with Type 1 diabetes,” Maria Grant, MD, leader of the research team and a professor in the University of Alabama at Birmingham Department of Ophthalmology and Visual Sciences, said in the UAB news release. Grant holds the Eivor and Alston Callahan, M.D., Endowed Chair in Ophthalmology.

The news release offered some background that is useful to understand Grant’s research.

First, it is known that Type 1 diabetes dysregulates the systemic renin-angiotensin system, or RAS. RAS is a system of hormones and enzymes that regulates blood pressure and other metabolic changes. Besides systemic RAS, there are also local RAS networks that act in diverse tissues. One key RAS enzyme is ACE2, or angiotensin-converting enzyme 2. The loss of ACE2 in diabetes activates the vasodeleterious RAS axis and lessens the vasoprotective RAS axis. Intriguingly, in a mouse model of Type 1 diabetes, feeding mice with a modified gut bacterial strain of Lactobacillus paracasei, which was engineered to produce human ACE2, protects the mice against diabetic retinopathy progression. Finally, lack of ACE2 in the gut was known to increase gut permeability and systemic inflammation.

The human studies, published in Prasad, Floyd et al. compared people with Type 1 diabetes versus controls. The subjects with Type 1 diabetes were further stratified into three groups: no diabetic retinopathy, non-proliferative diabetic retinopathy and the more serious disease called proliferative diabetic retinopathy. By measuring levels of certain immune cells and biomarkers in the blood, including gut microbial antigens, the researchers found that human subjects with retinopathy had a dysregulated systemic RAS and profound gut permeability defects that activated components of both the adaptive and innate immune response.

The UAB news release also noted that increases in the severity of diabetic retinopathy were found to correlate with increased levels of gut permeability biomarkers and a gut microbial antigen. This included increased levels of angiotensin II, the RAS hormone that activates the vasodeleterious RAS axis.

According to the UAB news release, using the Akita mouse-Type 1 diabetes model, researchers first gave the ACE2-producing Lactobacillus paracasei, developed by Qiuhong Li, PhD, from the University of Florida, to the mice orally beginning at the onset of diabetes. This probiotic treatment prevented the loss of gut epithelial ACE2 typically seen in Akita mice, and importantly, it prevented intestinal epithelial and endothelial barrier damage. It also reduced the high blood sugar levels known as hyperglycemia.

UAB noted that when the oral ACE2-producing Lactobacillus paracasei treatment was withheld until six months after diabetes was established, that delayed treatment reversed the gut barrier dysfunction and diabetic retinopathy that had already formed in the mice, including reducing the number of damaged capillaries in the retina.

Grant and colleagues, according to the news release, also found evidence for several mechanisms that contributed to the ACE2-reduced gut barrier damage and ACE2-lowering of blood sugar. To validate results from the Akita/ACE2-producing Lactobacillus paracasei model, they created a second model — a genetically modified Akita strain that overexpresses human ACE2 in small intestine epithelial cells.

“The significance of the work is we demonstrated that dysregulated intestinal RAS results in translocation of gut microbial antigens into the plasma,” Grant said in the news release.

Grant added that the bacterial peptides activate the endothelium via toll-like receptors, creating an inflammatory endothelium that has been strongly implicated in the pathogenesis of vascular diseases, including diabetic retinopathy.

“We demonstrated loss of intestinal barrier function in human subjects with Type 1 diabetes using gut barrier biomarkers, and this increase in permeability was associated with gut-derived immune cell activation,” she concluded in the news release.

Support came from National Institutes of Health grants EY012601, EY028858, EY028037, EY025383, EY032753, HL134640-01 and HL105349; Canadian Institutes of Health Research grant 883612; and Heart and Stroke Foundation grant 55542.

Reference

Ram Prasad; Jason L. Floyd; Mariana Dupont; Angela Harbour; Yvonne Adu-Agyeiwaah; Bright Asare-Bediako; Dibyendu Chakraborty; Kara Kichler; Aayush Rohella; Sergio Li Calzi; Michael E. Boulton; Regina Lammendella; Justin Wright; Gavin Y. Ouditl; Mohan K. Raizada; Bruce R. Stevens; Qiuhong Li. “Maintenance of enteral ACE2 prevents diabetic retinopathy in Type 1 diabetes.” Circulation Research. Published January 6, 2023. DOI: 10.1161/CIRCRESAHA.122.322003.